Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8c6d
Q-score: 0.614
Kingston NJ, Snowden JS, Martyna A, Shegdar M, Grehan K, Tedcastle A, Pegg E, Fox H, Macadam AJ, Martin J, Hogle JM, Rowlands DJ, Stonehouse NJ
bioRxiv (2023) [ DOI: doi:10.1101/2023.01.30.526315 Pubmed: 36778240 ]
- (2s,3r,4e)-2-aminooctadec-4-ene-1,3-diol (299 Da, Ligand)
- Genome polyprotein (fragment) (35 kDa, Protein from Enterovirus A71)
- Genome polyprotein (32 kDa, Protein from Enterovirus A71)
- Enterovirus a71 (Virus from Enterovirus A71)
- Genome polyprotein (26 kDa, Protein from Enterovirus A71)
VelcroVax tandem HBcAg with SUMO-Affimer inserted at MIR (T=4 VLP)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7zq8
Q-score: 0.612
Kingston NJ, Grehan K, Snowden JS, Hassall M, Alzahrani J, Paesen GC, Sherry L, Hayward C, Roe A, Stephen S, Tomlinson D, Zeltina A, Doores KJ, Ranson NA, Stacey M, Page M, Rose NJ, Bowden TA, Rowlands DJ, Stonehouse NJ
mSphere (2023) 8 pp. e0056822-e0056822 [ DOI: doi:10.1128/msphere.00568-22 Pubmed: 36719225 ]
VelcroVax tandem HBcAg with SUMO-Affimer inserted at MIR (T=3* VLP)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7zqa
Q-score: 0.513
Kingston NJ, Grehan K, Snowden JS, Hassall M, Alzahrani J, Paesen GC, Sherry L, Hayward C, Roe A, Stephen S, Tomlinson D, Zeltina A, Doores KJ, Ranson NA, Stacey M, Page M, Rose NJ, Bowden TA, Rowlands DJ, Stonehouse NJ
mSphere (2023) 8 pp. e0056822-e0056822 [ DOI: doi:10.1128/msphere.00568-22 Pubmed: 36719225 ]
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8ayx
Q-score: 0.649
Bahar MW, Nasta V, Fox H, Sherry L, Grehan K, Porta C, Macadam AJ, Stonehouse NJ, Rowlands DJ, Fry EE, Stuart DI
Commun Biol (2022) 5 pp. 1293-1293 [ DOI: doi:10.1038/s42003-022-04252-5 Pubmed: 36434067 ]
- Capsid protein, vp0 (37 kDa, Protein from Human poliovirus 3)
- Glutathione (307 Da, Ligand)
- Capsid protein, vp3 (26 kDa, Protein from Human poliovirus 3)
- Human poliovirus 3 (5 MDa, Virus from Human poliovirus 3)
- Capsid protein, vp1 (33 kDa, Protein from Human poliovirus 3)
- 1-[(3s)-5-[4-[(e)-ethoxyiminomethyl]phenoxy]-3-methyl-pentyl]-3-pyridin-4-yl-imidazolidin-2-one (410 Da, Ligand)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8ayy
Q-score: 0.647
Bahar MW, Nasta V, Fox H, Sherry L, Grehan K, Porta C, Macadam AJ, Stonehouse NJ, Rowlands DJ, Fry EE, Stuart DI
Commun Biol (2022) 5 pp. 1293-1293 [ DOI: doi:10.1038/s42003-022-04252-5 Pubmed: 36434067 ]
- Capsid protein, vp0 (37 kDa, Protein from Human poliovirus 3)
- Glutathione (307 Da, Ligand)
- Capsid protein, vp3 (26 kDa, Protein from Human poliovirus 3)
- Human poliovirus 3 (5 MDa, Virus from Human poliovirus 3)
- Capsid protein, vp1 (33 kDa, Protein from Human poliovirus 3)
- 3-{3,5-dimethyl-4-[3-(3-methyl-isoxazol-5-yl)-propoxy]-phenyl}-5-trifluoromethyl-[1,2,4]oxadiazole (381 Da, Ligand)
Poliovirus type 2 (strain MEF-1) virus-like particle in complex with capsid binder compound 17
Resolution: 1.88 Å
EM Method: Single-particle
Fitted PDBs: 8ayz
Q-score: 0.769
Bahar MW, Nasta V, Fox H, Sherry L, Grehan K, Porta C, Macadam AJ, Stonehouse NJ, Rowlands DJ, Fry EE, Stuart DI
Commun Biol (2022) 5 pp. 1293-1293 [ DOI: doi:10.1038/s42003-022-04252-5 Pubmed: 36434067 ]
- Human poliovirus 2 (5 MDa, Virus from Human poliovirus 2)
- Sphingosine (299 Da, Ligand)
- 4-[[4-[1,3-bis(oxidanylidene)isoindol-2-yl]phenyl]sulfonylamino]benzoic acid (422 Da, Ligand)
- Capsid protein, vp1 (33 kDa, Protein from Human poliovirus 2)
- Water (18 Da, Ligand)
- Capsid protein, vp3 (26 kDa, Protein from Human poliovirus 2)
- Capsid protein, vp0 (37 kDa, Protein from Human poliovirus 2)
Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8).
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8anw
Q-score: 0.62
Sherry L, Grehan K, Swanson JJ, Bahar MW, Porta C, Fry EE, Stuart DI, Rowlands DJ, Stonehouse NJ
Viruses (2022) 14 [ DOI: doi:10.3390/v14102159 Pubmed: 36298714 ]
- Capsid protein, vp0 (37 kDa, Protein from Human poliovirus 3)
- Sphingosine (299 Da, Ligand)
- Capsid protein, vp3 (26 kDa, Protein from Human poliovirus 3)
- Human poliovirus 3 (5 MDa, Virus from Human poliovirus 3)
- Capsid protein, vp1 (33 kDa, Protein from Human poliovirus 3)
Cryo-EM structure of fatty acid synthase (FAS) from Pichia pastoris
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7bc4
Q-score: 0.551
Snowden JS, Alzahrani J, Sherry L, Stacey M, Rowlands DJ, Ranson NA, Stonehouse NJ
Sci Rep (2021) 11 pp. 9773-9773 [ DOI: doi:10.1038/s41598-021-89196-2 Pubmed: 33963233 ]
- Flavin mononucleotide (456 Da, Ligand)
- Fatty acid synthase subunit alpha (206 kDa, Protein from Yeast)
- Fatty acid synthase (2 MDa, Complex from Komagataella phaffii GS115)
- Fatty acid synthase subunit beta (230 kDa, Protein from Yeast)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7bc5
Q-score: 0.381
Snowden JS, Alzahrani J, Sherry L, Stacey M, Rowlands DJ, Ranson NA, Stonehouse NJ
Sci Rep (2021) 11 pp. 9773-9773 [ DOI: doi:10.1038/s41598-021-89196-2 Pubmed: 33963233 ]
- Fatty acid synthase subunit alpha (206 kDa, Protein from Komagataella phaffii (strain GS115 / ATCC 20864))
- Fatty acid synthase (2 MDa, Complex from Komagataella phaffii GS115)
Chandler-Bostock R, Mata CP, Bingham RJ, Dykeman EC, Meng B, Tuthill TJ, Rowlands DJ, Ranson NA, Twarock R, Stockley PG
PLoS Pathog (2020) 16 [ Pubmed: 33370422 DOI: 10.1371/journal.ppat.1009146 ]
Image sets: